- Transaction Code: 3250-04
- CPT Code (Suggested): 83890, 83892, 83894, 83898, 83912
Patients testing positive for APC resistance or the Factor V Leiden mutation should be considered for testing for the Factor II (Prothrombin) G20210A variant. Individuals who are homozygous or heterozygous for the 20210A genotype have a 3-to 5-fold elevated risk for venous thrombosis.
The Method used for testing is PCR-RFLP. In brief, DNA is isolated from whole blood and subject to amplification with primers that recognize the polymorphic site. The amplified product is subjected to an enzyme which cleaves the amplified product at the mutation site and a gel electrophoresis enables a visual record of the results.
- Sample Required: 5 ml Whole Blood EDTA (purple top)
- Test Values: Homozygous normal (20210G); Heterozygous or Homozygous Abnormal (20210A)
- Analytic Time: Run weekly (Tuesdays) on samples received Wednesday through Monday noon; abnormal tests are confirmed by repeating tests.
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