- About Our Research
- Meet Our Researchers
- Jing-Fei Dong, M.D., Ph.D.
- Xiaoyun Fu, Ph.D.
- William E. Hobbs, M.D., Ph.D.
- Jill M. Johnsen, M.D.
- Neil C. Josephson, M.D.
- Barbara A. Konkle, M.D.
- José A. López, M.D.
- Karen Nelson, Ph.D. D(ABHI)
- Kathleen Pratt, Ph.D.
- Sherrill Slichter, M.D.
- Paul Warner, Ph.D.
- Timothy R. Watkins, M.D., MSc
- Core Facilities
- Videos
- Fu X, Kassim SY, Parks WC, Heinecke JW: Hypochlorous acid oxigenates the cysteine switch domain of pro-matrilysin (MMP-7): A mechanism for matrix metalloproteinase activation and atherosclerotic plaque rupture by myeloperoxidase. J Biol Chem 276(44):41279-41287, 2001. PMID11533038
- Fu X, Mueller DM, Heinecke JW: Generation of intramolecular and intermolecular sulfenamides, sulfinamide, and sulfinamide by hypochlorous acid: A potential pathway for oxidative cross-linking of low-density lipoprotein by myeloperoxidase. Biochemistry 41(4):1293-1301, 2002. PMID11802729
- Fu X, Kassim SY, Parks WC, Heinecke JW: Hypochlorous acid oxygenated by myeloperoxidase modifies adjacent tryptophan and glycine residues in catalytic domain of matrix metalloproteinase-7 (Matrilysin). J Biol Chem 278:28403-28409, 2003. PMID12759346
- Fu X, Kao J, Bergt C, Kassim SY, Huq NP, d'Avignon A, Parks WC, Mecham RP, Heinecke JW: Oxidative cross-linking of tryptophan to glycine restrains matrix metalloproteinase activity. J Biol Chem 279:6209-6212, 2004. PMID14670964
- Bergt C, Fu X, Huq, NP, Kao J, Heinecke JW: Lysine residues direct the chlorination of tyrosines in YXXK motifs of apolipoprotein A-I when hypochlorous acid oxidizes high density lipoprotein. J Biol Chem 279:7856-7866, 2004. PMID14660678
- Shao B, Belaaouaj A, Verlinde CL, Fu X, Heinecke JW. Methionine sulfoxide and proteolytic cleavage contribute to the inactivation of cathepsin G by hypochlorous acid: An oxidative mechanism for regulation of serine proteinases by myeloperoxidase. J. Biol Chem. 280(32) 29311-21, 2005. PMID15967795
- Kassim SY, Fu X, Liles WC, Shapiro SD, Parks WC, Heinecke JW: NADPH Oxidase Restrains the Matrix Metalloproteinase Activity of Macrophages. J. Biol. Chem. 280(34): 30201-5, 2005. PMID15983040
- Shao B, Fu X, Mecdonald TO, Green PS, Uchida K, O'Brien KD, Oram JF, Heinecke JW: Acrolein Impairs ABCA1-dependent Cholesterol Export from Cells through Site-Specific Modification of Apolipoprotein A-I. J. Biol. Chem. 280(43):36386-96, 2005. PMID16126721
- Fu X, Wang Y, Kao J, Irwin A,d'Avignon A, Mecham RP, Parks WC, Heinecke JW: Specific Sequence Motifs Direct the Oxygenation and Chlorination of Tryptophan by Myeloperoxidase. Biochemistry 459(12), 3961-71, 2006. PMID16548523
- Wang Y, Rosen H, Madtes DK, Shao B, Martin TR, Heinecke JW, Fu X: Myeloperoxidase inactivates TIMP-1 by oxidizing its N-terminal cysteine residue: An oxidative mechanism for regulating proteolysis during inflammation. J. Biol. Chem. 282(44):31826-34, 2007. PMID17726014
- Fu X, Gharib SA, Green PS, Aitken ML, Frazer DA, Park DR, Vaisar T, Heinecke JW: Spectral Index for Assessment of Differential Protein Expression in Shotgun Proteomics. J. Proteome Res. 7(3) 845-854, 2008. PMID18198819
- Gharib SA, Green PS, Aitken ML, Frazer DA, Park DR, Vaisar T, Heinecke JW, Fu X: Mapping the lung proteome in Cystic Fibrosis. J. Proteome Res. 8(6):3020-8, 2009. PMID19354268
- Yuan W, Wang Y, Heinecke JW, Fu X: Hypochlorous acid converts the γ-glutamyl group of glutathione disulfide to 5-hydroxy-butyrolactam: a potential marker for neutrophil activation. J. Biol. Chem. 284(39):26908-17, 2009. PMID19584048
- Rosen H, Klebanoff SJ, Wang Y, Brot N, Heinecke JW, Fu X: Methionine Oxidation Contributes to Bacterial Killing by the Myeloperoxidase System of Neutrophils, Proc. Natl. Acad. Sci. USA, 106(44):18686-91, 2009. PMID19833874
- Chen J, Fu X, Wang Y, Ling M, McMullen B, Kulman J, Chung D, López JA: Oxidative modification of von Willebrand factor by neutrophil oxidants inhibits its cleavage by ADAMTS13. Blood, 115(3) 706-12, 2010. PMID19812385
- Chen J, Reheman A, Gushiken FC, Nolascol L, Fu X, Moake JL, Ni H, López JA: N-acetylcysteine reduces the size and activity of von Willebrand factor in human plasma and mice. J. Clin. Invest, 121(2):593-603, 2011. PMID21266777
- Herrero R, Kajikawa O, Matute-Bello G, Wang Y, Hagimoto N, Mongovin S, Wong V, Park DR, Brot N, Heinecke JW, Rosen H, Goodman RB, Fu X, Martin TR, The biological Activity of FasL in humans and mice is determined by the structure of its stalk region. J Clin. Invest, 121(3):1174-90, 2011. PMID21285513
- Fu X, Chen J, Gallagher R, Zheng Y, Chung DW, López JA: Shear stress-induced unfolding of von Willebrand factor accelerates oxidation of key methionine residues in the A1A2A3 region. Blood. 118(19) 5283-91, 2011. PMID21917758